Tipificación de aislamientos de Ralstonia mannitolilytica en un hospital pediátrico de la Ciudad de México

  • Alejandra Aquino-Andrade INSTITUTO NACIONAL DE PEDIATRÍA
  • Jocelin Merida-Vieyra INSTITUTO NACIONAL DE PEDIATRÍA
  • Antonino Lara-Hernández INSTITUTO NACIONAL DE PEDIATRÍA
  • Hilda Hernández-Orozco INSTITUTO NACIONAL DE PEDIATRÍA
  • Agustin De Colsa-Ranero INSTITUTO NACIONAL DE PEDIATRÍA

Resumen

Introducción: Ralstonia mannitolilytica es un agente causal de infecciones asociadas al cuidado de la salud. Las especies del género Ralstonia son difíciles de identificar y diferenciar. Nuestro objetivo fue identificar y tipificar aislamientos de R. mannitolilytica obtenidos de hemocultivos de pacientes pediátricos. Métodos: Colectamos 10 aislamientos de R. mannitolilytica identificados con un sistema automatizado, un sistema de espectrometría de masas con analizador de tiempo de vuelo y desorción mediante láser asistida por matriz (MALDI-TOF-MS, Matrix-assisted laser desorption/ionization time of flight mass spectrometry) y análisis del gen 16S rRNA. Se determinó el perfil de resistencia y se tipificaron las carbapenemasas a través de PCR y secuenciación. Se estableció la relación clonal de los aislamientos mediante electroforesis en gel de campos pulsados (PFGE). Resultados: El sistema MALDI-TOF-MS y análisis del gen 16S rRNA identificaron a los aislamientos como R. mannitolilytica; todos pertenecieron a la misma clona y fueron resistentes a meropenem, se detectaron a las carbapenemasas OXA-443 y OXA-444 en todos ellos. Conclusiones: Este es el primer reporte de aislamientos de R. mannitolilytica con origen clonal y que poseen las carbapenemasas OXA-443 y OXA-444 en pacientes pediátricos mexicanos.

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Publicado
2022-04-25
Cómo citar
Aquino-Andrade, A., Merida-Vieyra, J., Lara-Hernández, A., Hernández-Orozco, H., & De Colsa-Ranero, A. (2022). Tipificación de aislamientos de Ralstonia mannitolilytica en un hospital pediátrico de la Ciudad de México. Acta Pediátrica De México, 43(2), 73-78. https://doi.org/10.18233/APM43No2pp73-782392
Sección
Artículo original